培养条件、he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM) 10 ng/ml cholera toxin 0.005 mg/ml insulin 0.005 mg/ml transferrin 100 ng/ml hydrocortisone 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311) Fetal Bovine Serum, 10% final conc (ATCC 30-2020)