培养条件、he base medium for this cell line is: 50% ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. 50% MEGM (Mammary Epithelial Growth Medium from Clonetics/Lonza (MEGM Bullet Kit; CC-3150) made of MEBM basal medium and SingleQuot additives (ATCC does not use gentamycin-amphotericin B).Note: Do not filter complete medium.To make the final complete growth medium add the following components to the base medium: fetal bovine serum to a final concentration of 3%. 建议血清浓度5%